Epernicus: Stephen Spiro, Ph.D.

Stephen Spiro, Ph.D.

Position:
Associate Professor

Molecular and Cell Biology

University of Texas at Dallas (Richardson, TX)
Degrees:

Ph.D., Microbiology, University of Sheffield (Sheffield, UK)

B.Sc., Molecular Biology, University of Edinburgh (Edinburgh, UK)
Past Advisors:

John R. Guest (as Post Doctoral Fellow)
Research:
Other Experience:
2004-2006 Associate Professor, Georgia Institute of Technology

1991-2003 Lecturer, Senior Lecturer, Reader, University of East Anglia

Communities:

Stephen Spiro's Genealogy

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Stephen Spiro's Publications (31)

Escherichia coli NsrR regulates a pathway for the oxidation of 3-nitrotyramine to 4-hydroxy-3-nitrophenylacetate.
Rankin LD, Bodenmiller DM, Partridge JD, Nishino SF, Spain JC, Spiro S

Journal of bacteriology. 2008 Sep;190(18):6170-7
A combination of cytochrome c nitrite reductase (NrfA) and flavorubredoxin (NorV) protects Salmonella enterica serovar Typhimurium against killing by NO in anoxic environments.
Mills PC, Rowley G, Spiro S, Hinton JC, Richardson DJ

Microbiology (Reading, England). 2008 Apr;154(Pt 4):1218-28
Analysis of the nitric oxide-sensing non-heme iron center in the NorR regulatory protein.
Tucker NP, D'Autréaux B, Yousafzai FK, Fairhurst SA, Spiro S, Dixon R

The Journal of biological chemistry. 2008 Jan 11;283(2):908-18
Characterization of the nitric oxide-reactive transcriptional activator NorR.
D'Autréaux B, Tucker N, Spiro S, Dixon R

Methods in enzymology. 2008;437:235-51
Escherichia coli cytochrome c nitrite reductase NrfA.
Clarke TA, Mills PC, Poock SR, Butt JN, Cheesman MR, Cole JA, Hinton JC, Hemmings AM, Kemp G, Söderberg CA, Spiro S, Van Wonderen J, Richardson DJ

Methods in enzymology. 2008;437:63-77
Genome-wide identification of binding sites for the nitric oxide-sensitive transcriptional regulator NsrR.
Efromovich S, Grainger D, Bodenmiller D, Spiro S

Methods in enzymology. 2008;437:211-33
The NsrR regulon of Escherichia coli K-12 includes genes encoding the hybrid cluster protein and the periplasmic, respiratory nitrite reductase.
Filenko N, Spiro S, Browning DF, Squire D, Overton TW, Cole J, Constantinidou C

Journal of bacteriology. 2007 Jun;189(12):4410-7
Regulators of bacterial responses to nitric oxide.
Spiro S

FEMS microbiology reviews. 2007 Mar;31(2):193-211
Transcription factor NNR from Paracoccus denitrificans is a sensor of both nitric oxide and oxygen: isolation of nnr* alleles encoding effector-independent proteins and evidence for a haem-based sensing mechanism.
Lee YY, Shearer N, Spiro S

Microbiology (Reading, England). 2006 May;152(Pt 5):1461-70
Cell-cell signaling in Xanthomonas campestris involves an HD-GYP domain protein that functions in cyclic di-GMP turnover.
Ryan RP, Fouhy Y, Lucey JF, Crossman LC, Spiro S, He YW, Zhang LH, Heeb S, Cámara M, Williams P, Dow JM

Proceedings of the National Academy of Sciences of the United States of America. 2006 Apr 25;103(17):6712-7
Mechanism of transcriptional regulation by the Escherichia coli nitric oxide sensor NorR.
Tucker NP, D'autréaux B, Spiro S, Dixon R

Biochemical Society transactions. 2006 Feb;34(Pt 1):191-4
Nitric oxide-sensing mechanisms in Escherichia coli.
Spiro S

Biochemical Society transactions. 2006 Feb;34(Pt 1):200-2
The yjeB (nsrR) gene of Escherichia coli encodes a nitric oxide-sensitive transcriptional regulator.
Bodenmiller DM, Spiro S

Journal of bacteriology. 2006 Feb;188(3):874-81
A non-haem iron centre in the transcription factor NorR senses nitric oxide.
D'Autréaux B, Tucker NP, Dixon R, Spiro S

Nature. 2005 Sep 29;437(7059):769-72
Detoxification of nitric oxide by the flavorubredoxin of Salmonella enterica serovar Typhimurium.
Mills PC, Richardson DJ, Hinton JC, Spiro S

Biochemical Society transactions. 2005 Feb;33(Pt 1):198-9
DNA binding properties of the Escherichia coli nitric oxide sensor NorR: towards an understanding of the regulation of flavorubredoxin expression.
Tucker N, D'autréaux B, Spiro S, Dixon R

Biochemical Society transactions. 2005 Feb;33(Pt 1):181-3
Point mutations in the DNA- and cNMP-binding domains of the homologue of the cAMP receptor protein (CRP) in Mycobacterium bovis BCG: implications for the inactivation of a global regulator and strain attenuation.
Spreadbury CL, Pallen MJ, Overton T, Behr MA, Mostowy S, Spiro S, Busby SJ, Cole JA

Microbiology (Reading, England). 2005 Feb;151(Pt 2):547-56
DNA binding activity of the Escherichia coli nitric oxide sensor NorR suggests a conserved target sequence in diverse proteobacteria.
Tucker NP, D'Autréaux B, Studholme DJ, Spiro S, Dixon R

Journal of bacteriology. 2004 Oct;186(19):6656-60
Construction of a whole-cell gene reporter for the fluorescent bioassay of nitrate.
Taylor CJ, Bain LA, Richardson DJ, Spiro S, Russell DA

Analytical biochemistry. 2004 May 1;328(1):60-6
Protein-template-driven formation of polynuclear iron species.
Malone SA, Lewin A, Kilic MA, Svistunenko DA, Cooper CE, Wilson MT, Le Brun NE, Spiro S, Moore GR

Journal of the American Chemical Society. 2004 Jan 21;126(2):496-504
The ferric uptake regulator of Pseudomonas aeruginosa has no essential cysteine residues and does not contain a structural zinc ion.
Lewin AC, Doughty PA, Flegg L, Moore GR, Spiro S

Microbiology (Reading, England). 2002 Aug;148(Pt 8):2449-56
The NorR protein of Escherichia coli activates expression of the flavorubredoxin gene norV in response to reactive nitrogen species.
Hutchings MI, Mandhana N, Spiro S

Journal of bacteriology. 2002 Aug;184(16):4640-3
Spectral properties of bacterial nitric-oxide reductase: resolution of pH-dependent forms of the active site heme b3.
Field SJ, Prior L, Roldan MD, Cheesman MR, Thomson AJ, Spiro S, Butt JN, Watmough NJ, Richardson DJ

The Journal of biological chemistry. 2002 Jun 7;277(23):20146-50
Transcription factor FnrP from Paracoccus denitrificans contains an iron-sulfur cluster and is activated by anoxia: identification of essential cysteine residues.
Hutchings MI, Crack JC, Shearer N, Thompson BJ, Thomson AJ, Spiro S

Journal of bacteriology. 2002 Jan;184(2):503-8
Two conserved glutamates in the bacterial nitric oxide reductase are essential for activity but not assembly of the enzyme.
Butland G, Spiro S, Watmough NJ, Richardson DJ

Journal of bacteriology. 2001 Jan;183(1):189-99

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Stephen Spiro, Ph.D.

Associate Professor

Stephen Spiro's Genealogy

Stephen Spiro's Publications (31)