Research Scientist I

Biotechnology and Bioengineering Center

Medical College of Wisconsin (Milwaukee, WI)

My research focuses on the regulation of cellular energy metabolism and the physical biology of cellular signaling proteins.

My research addresses questions on transport processes and energy metabolism of cells using a combination of computational models and model driven experiments. In my recent research I have specifically addressed questions on 1) localization, diffusion, binding and membrane-exchange processes of Ras2 a signaling protein in yeast relevant to cancer; 2) defining metabolic phenotypes (glycolysis and ATP hydrolysis rates) of resting muscles; and 3) identifying catalytic mechanisms of individual enzymes and transporters constituting the metabolic pathways of interest. My computational approach is based on detailed kinetic modeling of individual enzymes and transporters, which are then built into a differential equation (ordinary or partial) based pathway model parameterized and validated by independent experimental data sets. My experimental approach, which is guided by computer model based experiment design, includes characterization of individual enzyme kinetics and whole organelle kinetics. The overall goal is to be able to synthesize information on individual parts into a predictive model of system behavior in normal and pathological states.

2004-2005 American Heart Association (Pacific Mountain Affiliate) Predoctoral Fellowship

2004 American Heart Association (Pacific Mountain Affiliate) Outstanding Predoctoral Fellow