Epernicus: Didier Busso, Ph.D.

Didier Busso, Ph.D.

Position:
Research Assistant

Structural Biology

Institute of Genetics and Molecular and Cellular Biology (Strasbourg, France)
Advisor:
Dino Moras
Degrees:

Ph.D., Molecular Biology, University of Strasbourg (Strasbourg, France)
Past Advisors:

Sung-Hou Kim (as Post Doctoral Fellow)

Jean-Marc Egly (as Graduate Student - Ph.D.)

Claude Gigot (as Graduate Student - Masters)
Research:
My research consists on establishing high-throughput methods for cloning, protein expression, purification and crystallisation.

Communities:

Didier Busso's Genealogy

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Didier Busso's Publications (22)

TCTP protects from apoptotic cell death by antagonizing bax function.
Susini L, Besse S, Duflaut D, Lespagnol A, Beekman C, Fiucci G, Atkinson AR, Busso D, Poussin P, Marine JC, Martinou JC, Cavarelli J, Moras D, Amson R, Telerman A

Cell death and differentiation. 2008 Aug;15(8):1211-20
A comparison of inoculation methods to simplify recombinant protein expression screening in Escherichia coli.
Busso D, Stierlé M, Thierry JC, Moras D

BioTechniques. 2008 Jan;44(1):101-6
Automated recombinant protein expression screening in Escherichia coli.
Busso D, Stierlé M, Thierry JC, Moras D

Methods in molecular biology (Clifton, N.J.). 2008;426:175-86
The structural biology and genomics platform in strasbourg: an overview.
Busso D, Thierry JC, Moras D

Methods in molecular biology (Clifton, N.J.). 2008;426:523-36
Co-expression of protein complexes in prokaryotic and eukaryotic hosts: experimental procedures, database tracking and case studies.
Romier C, Ben Jelloul M, Albeck S, Buchwald G, Busso D, Celie PH, Christodoulou E, De Marco V, van Gerwen S, Knipscheer P, Lebbink JH, Notenboom V, Poterszman A, Rochel N, Cohen SX, Unger T, Sussman JL, Moras D, Sixma TK, Perrakis A

Acta crystallographica. Section D, Biological crystallography. 2006 Oct;62(Pt 10):1232-42
Eukaryotic expression: developments for structural proteomics.
Aricescu AR, Assenberg R, Bill RM, Busso D, Chang VT, Davis SJ, Dubrovsky A, Gustafsson L, Hedfalk K, Heinemann U, Jones IM, Ksiazek D, Lang C, Maskos K, Messerschmidt A, Macieira S, Peleg Y, Perrakis A, Poterszman A, Schneider G, Sixma TK, Sussman JL, Sutton G, Tarboureich N, Zeev-Ben-Mordehai T, Jones EY

Acta crystallographica. Section D, Biological crystallography. 2006 Oct;62(Pt 10):1114-24
Implementation of semi-automated cloning and prokaryotic expression screening: the impact of SPINE.
Alzari PM, Berglund H, Berrow NS, Blagova E, Busso D, Cambillau C, Campanacci V, Christodoulou E, Eiler S, Fogg MJ, Folkers G, Geerlof A, Hart D, Haouz A, Herman MD, Macieira S, Nordlund P, Perrakis A, Quevillon-Cheruel S, Tarandeau F, van Tilbeurgh H, Unger T, Luna-Vargas MP, Velarde M, Willmanns M, Owens RJ

Acta crystallographica. Section D, Biological crystallography. 2006 Oct;62(Pt 10):1103-13
Recombinant protein expression and solubility screening in Escherichia coli: a comparative study.
Berrow NS, Büssow K, Coutard B, Diprose J, Ekberg M, Folkers GE, Levy N, Lieu V, Owens RJ, Peleg Y, Pinaglia C, Quevillon-Cheruel S, Salim L, Scheich C, Vincentelli R, Busso D

Acta crystallographica. Section D, Biological crystallography. 2006 Oct;62(Pt 10):1218-26
Cyclin H binding to the RARalpha activation function (AF)-2 domain directs phosphorylation of the AF-1 domain by cyclin-dependent kinase 7.
Bour G, Gaillard E, Bruck N, Lalevée S, Plassat JL, Busso D, Samama JP, Rochette-Egly C

Proceedings of the National Academy of Sciences of the United States of America. 2005 Nov 15;102(46):16608-13
Construction of a set Gateway-based destination vectors for high-throughput cloning and expression screening in Escherichia coli.
Busso D, Delagoutte-Busso B, Moras D

Analytical biochemistry. 2005 Aug 15;343(2):313-21
Structural genomics of eukaryotic targets at a laboratory scale.
Busso D, Poussin-Courmontagne P, Rosé D, Ripp R, Litt A, Thierry JC, Moras D

Journal of structural and functional genomics. 2005;6(2-3):81-8
Structural and functional characterization of a novel phosphodiesterase from Methanococcus jannaschii.
Chen S, Yakunin AF, Kuznetsova E, Busso D, Pufan R, Proudfoot M, Kim R, Kim SH

The Journal of biological chemistry. 2004 Jul 23;279(30):31854-62
Structure of OsmC from Escherichia coli: a salt-shock-induced protein.
Shin DH, Choi IG, Busso D, Jancarik J, Yokota H, Kim R, Kim SH

Acta crystallographica. Section D, Biological crystallography. 2004 May;60(Pt 5):903-11
Using an Escherichia coli cell-free extract to screen for soluble expression of recombinant proteins.
Busso D, Kim R, Kim SH

Journal of structural and functional genomics. 2004;5(1-2):69-74
Structure of the hypothetical protein AQ_1354 from Aquifex aeolicus.
Oganesyan V, Busso D, Brandsen J, Chen S, Jancarik J, Kim R, Kim SH

Acta crystallographica. Section D, Biological crystallography. 2003 Jul;59(Pt 7):1219-23
Expression of soluble recombinant proteins in a cell-free system using a 96-well format.
Busso D, Kim R, Kim SH

Journal of biochemical and biophysical methods. 2003 Mar 28;55(3):233-40
Crystal structure of a stress inducible protein from Mycoplasma pneumoniae at 2.85 A resolution.
Choi IG, Shin DH, Brandsen J, Jancarik J, Busso D, Yokota H, Kim R, Kim SH

Journal of structural and functional genomics. 2003;4(1):31-4
Solution structure of the N-terminal domain of the human TFIIH MAT1 subunit: new insights into the RING finger family.
Gervais V, Busso D, Wasielewski E, Poterszman A, Egly JM, Thierry JC, Kieffer B

The Journal of biological chemistry. 2001 Mar 9;276(10):7457-64
Distinct regions of MAT1 regulate cdk7 kinase and TFIIH transcription activities.
Busso D, Keriel A, Sandrock B, Poterszman A, Gileadi O, Egly JM

The Journal of biological chemistry. 2000 Jul 28;275(30):22815-23
Reconstitution of the transcription factor TFIIH: assignment of functions for the three enzymatic subunits, XPB, XPD, and cdk7.
Tirode F, Busso D, Coin F, Egly JM

Molecular cell. 1999 Jan;3(1):87-95
Expression in Escherichia coli: purification and characterization of cyclin H, a subunit of the human general transcription/DNA repair factor TFIIH.
Poterszman A, Andersen G, Busso D, Rossignol M, Egly JM, Thierry JC

Protein expression and purification. 1997 Mar;9(2):153-8
The structure of cyclin H: common mode of kinase activation and specific features.
Andersen G, Busso D, Poterszman A, Hwang JR, Wurtz JM, Ripp R, Thierry JC, Egly JM, Moras D

The EMBO journal. 1997 Mar 3;16(5):958-67

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Didier Busso, Ph.D.

Research Assistant

My research consists on establishing high-throughput methods for cloning, protein expression, purification and crystallisation.

Didier Busso's Genealogy

Didier Busso's Publications (22)