Research Fellow

Developmental and Molecular Biology

Albert Einstein College of Medicine (Bronx, New York)

Richard Stanley

Molecular and biochemical basis of cell signaling by the metalloproteases TACE and ADAM10

ADAM metalloproteinases are the major enzymes responsible for catalyzing the release of membrane anchored proteins (such as growth factors, cytokines, and chemokines) from the cell surface in metazoan organisms. As a consequence of their pivotal roles in cellular processes such as proliferation, differentiation, migration and apoptosis, regulation of their activity is complex and multi-layered. As a post-doctoral researcher in Dr. Turk’s lab, I have defined the molecular basis of the active site contribution to substrate recognition by ADAMs. The release of cytokines and growth factors from cells is tightly regulated in vivo, yet the signaling pathways leading to activation of ADAMs are poorly defined. I am currently taking two approaches to better characterize these pathways. One approach focuses on using tandem affinity purification (TAP) to identify proteins associated with TACE in the macrophage cell line RAW264.7.My second approach is to investigate candidate molecules likely to be involved in the regulation of ADAM-dependent ectodomain shedding.

2004-2005 Assistant Professor of Biochemistry at the University “Al. I. Cuza”, Iasi, Romania.

2001-2003 Teaching Assistant at the “Ecole Polytechnique Universitaire de Lille”, France.

2000-2004 Doctoral Research Training Grant from AUF (Agence Universitaire de la Phrancophonie, France)

1999-2000 1. Pre-doctoral Training Scholarship from the European Program ERASMUS-SOCRATES